RT Journal
T1 GENETIC DIVERSITY AMONG COMMON LEOPARDS INHABITING AZAD JAMMU AND KASHMIR, PAKISTAN
A1 S. S. Bibi
A1 A. Rehman
A1 R. A. Minhas
A1 S. Andleeb
A1 M. S. Awan
A1 K. William
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 25
IS 3
SP 148
OP 154
YR 2015
FD 2015/06/01
DO DOI NA
AB The common leopard (Panthera pardus) is one of the highly threatened species of big cats in Pakistan. Azad Jammu and Kashmir (AJK) is among the main distribution areas of these leopards but due to poaching, habitat fragmentation and other anthropogenic activities, there is a rapid decline of the population in the state. We carried out the study to analyze the genetic diversity of common leopard in Azad Jammu and Kashmir (AJK).We used skin samples that were collected from the killed leopards by farmers and local hunters from six different localities of AJK. We used phenol chloroform triton modified method to extract the DNA and used four SSR markers (FCA043, FCA026, FCA075 and FCA090) to analyze genetic diversity by using PCR-based simple sequence repeat (SSR) analysis. We also calculated Allelic frequencies, Nei’s genetic diversities index (within and among populations) and Shannon information index with POPGENE 32 program which showed that highest genetic distance (0.4275) was established between the Kotli (Pop-6) and Hattian (Pop-3) populations, and lowest between Hattian (Pop-3) and Neelum (Pop-2) populations (0.0393) of common leopard. Mean gene flow (Nm) was found to be 0.8030 which showed low level of gene flow between different populations and higher level genetic differentiation between populations supported our hypothesis of habitat fragmentation. According to Luikart et al. (1998) for
K1 SSR analysis, gene flow, genetic distance.
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-621