RT Journal
T1 EFFECTS OF ARGININE ON CYTOKINES AND NITRIC OXIDE SYNTHESIS IN BROILERS
A1 Y. W. Guo
A1 B. L. Shi
A1 S. M. Yan
A1 Y. Q. Xu
A1 J. L. Li
A1 T. Y. Li
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 25
IS 2
SP 366
OP 371
YR 2015
FD 2015/04/01
DO DOI NA
AB <p>The effect of dietary supplement of arginine on immunoglobulin, cytokine and nitric oxide (NO) synthesis in broilers was investigated. Three hundred one-day-old Arbor Acre mixed-sex broiler chickens were randomly allotted to five dietary treatments with six replicates in each treatment and ten chickens within each replicate. The five treatments viz., the basal diet with 0 (arginine-deficient group), 4.5 (arginine-normal group, control), 9.0, 13.5,and 18.0 g/kg arginine. The feeding experiment period was 42 days. The results of&nbsp;<em>in vivo</em>experiments showed that with increasing levels of dietary arginine, interleukin (IL)-2, NO content and inducible nitric oxide synthase (iNOS) activity in serum as well as iNOS mRNA expression in the ileum and liver of broiler chickens increasedquadratically at the age of 21 d(<em>P&lt;0.05</em>).In addition, positive effects of arginine tended to be suppressed when addition of arginine in the diet was increased to 18.0 g/kg. The results of&nbsp;<em>in vitro</em>&nbsp;experiment indicated that, IL-2 and iNOS content increased quadratically with increasing addition of arginine (<em>P&lt;0.01</em>), and 80 &mu;g/mL arginine supplement group had the highest value. Dietary arginine improved the levels of IL-2, NO and iNOS expression in a quadratic dose-dependent manner.</p>
K1 Arginine, Broiler, Cytokine, Nitric Oxide Synthase, mRNA Expression
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-52