RT Journal T1 DNA CLONING AND MOLECULAR CHARACTERIZATION OF H2A AND H4 GENES IN PEPPER A1 Ming-Hua Deng A1 Hui Zhou A1 Jin-Long Huo A1 Jun-Heng Lv A1 Jin-Fen Wen A1 Hai-Shan Zhu JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 25 IS 1 SP 278 OP 283 YR 2015 FD 2015/02/01 DO DOI NA AB

The complete coding sequences (CDS) of “Yunnan Purple Pepper No.1” (Capsicum annuum L.) H2A and H4 genes were amplified using the reverse transcriptase polymerase chain reaction based on the conserved sequence information of some Solanaceae plants and known highly homologous pepper ESTs. The nucleotide sequences analysis of these two genes revealed that pepper H2A gene encodes a protein of 142 amino acids that has high homology with the H2A-like protein of 5 species: matrimony vine, grape, alfalfa, arabidopsis and maize. Sequence analysis of the second gene revealed that the pepper H4 encodes a protein of 103 amino acids that has high homology with the proteins of 4 species: tomato, tobacco, eggplant and petunia. The tissue expression analysis indicated that the pepper H2A and H4 genes were moderately expressed in the seven tissues. Our experiment established the primary foundation for further research on these two pepper genes.

K1 Capsicum annuum L.; H2A; H4; Gene expression profile, Bioinformatics analysis PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-37