RT Journal T1 OPTIMIZATION OF ULTRASOUND-ASSISTED EXTRACTION CONDITIONS OF ANTIOXIDATIVE COMPONENTS FROM ARUNDINA GRAMINIFOLIA BY RESPONSE SURFACE METHODOLOGY BASED ON DPPH RADICAL SCAVENGING ASSAY A1 X. F. Li A1 Y. T. Gao A1 X. X. Pang A1 X. F. Liu A1 W. Y. Li A1 Y. L. Zhang A1 B. Y. Fan JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 25 IS 3 SP 254 OP 259 YR 2015 FD 2015/06/01 DO DOI NA AB

Response surface methodology(RSM) was employed to optimize conditions for ultrasound-assisted extraction(UAE) of antioxidantive components from Arundinagraminifolia. 1,1-diphenyl-2-picryl-hydrazyl(DPPH) free radical scavenging assay was used to evaluate the activity of antioxidative components. Based on the single-factor test, we identified extraction time, ethanol volume fraction, extraction temperature and liquid-solid ratio as the main variables that influence DPPH free radical scavenging activity of A. graminifolia extract. The optimal conditions to achieve the maximum activity were determined as follows: extraction time 35 min, extraction temperature46ºC, ethanol volume fraction 74%and liquid-solidratio27 mL.g -1 . The scavenging rateof 78.71%was achieved under the optimal extraction conditions, which was well in agreement with the optimal predicted values (79.16%). This extraction method was simple and efficient and provided a method of sample preparation to determine DPPH radical scavenging activity of total antioxidative components from A. graminifolia. The research also provided a reference for full utilization of A. graminifolia and identified a technique to extract antioxidative components.

K1 Arundinagraminifolia, antioxidative components, ultrasound-assisted extraction, response surface methodology, DPPH radical scavenging gassay PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-338