RT Journal
T1 TISSUE CULTURE AND PLANT REGENERATION OF PRUNUS CAMPANULATA
A1 Y. H. Zhang
A1 J. D. Rong
A1 Y. Fu
A1 L. G. Chen
A1 L. Y. Chen
A1 Y.S. Zheng
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 25
IS 3
SP 146
OP 151
YR 2015
FD 2015/06/01
DO DOI NA
AB <p>This study used the tender shoot of Prunus campanulata Maxim. of the current year as explants for the induction and rapid propagation of adventitious buds. The results showed that the bud induction rate peaked at 94.44% after treatment with Murashige and Skoog medium (MS) +2.0 mg L-1 6-benzyladenine (6-BA) + 0.1 mg L-1 naphthalene acetic acid (NAA). The multiplication coefficient was 7.44 after treatment with MS +3.0 mg L-1 6-BA + 0.01 mg L-1 NAA + 30 g L-1 sucrose. The combination of MS + 0.01 mg L-1 NAA + 0.3 mg L-1 gibberellic acid had the best growth-promoting effects (seedlings grew well; 2-4 cm high). The treatment of 1/2 MS + 1.0 mg L-1 indole-3-butyric acid + 0.5 mg L-1 IAA + 20 g L -1 sucrose had a rooting percentage of 82.35% in which the plants grew well with fast rooting and lengths of 3-4 cm. Treatment consisting of 1/2 MS +1.5 mg L-1 ABT one rooting powder (ABT1) + 20 g L-1 sucrose had the highest rooting percentage (93.05%) and growth. The matrix of vermiculite and perlite (1:1) was optimal for transplantation with a survival rate of up to 94.07%. This study involved the optimal mediums and culture conditions, all of which were significant for industrialized breeding and seedling production.</p>
K1 Prunus campanulata Maxim., tissue culture, micropropagation
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-322