RT Journal T1 EFFICIENT INDUCTION, PROLIFERATION AND REGENERATION OF RUBBER TREE (H EVEA BRASILIENSIS MUELL. ARG) VIA SOMATIC EMBRYOGENESIS A1 H. Zhao A1 R. Z. Jia A1 Y. J. Zhu A1 A. P. Guo A1 H. C. Zeng A1 M. Peng JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 25 IS 3 SP 134 OP 140 YR 2015 FD 2015/06/01 DO DOI NA AB

An efficient protocol has been established to induce and proliferateembryogenic callus and regenerate plants using anther tissue culture of rubber tree (Heveabrasiliensis Muell. Arg), clone Reyan 7-33-97. The process of embryo-development from somatic embryos was divided into five stages: embryogenic callus, globular embryos, heart-shaped embryos, torpedo embryos and cotyledon embryos. Efficiency of proliferation and regeneration using embryonic tissues at different physiological stages was determined. A number of conclusions were reached. Friable embryogenic callus was the most suitable tissue for direct long-term proliferation by subsequent subcultures. The rates of proliferation and embryo development did not decrease after two years in tissue culture. Heart-shaped embryos and early embryogenic forms, including embryogenic callus and globular embryos, could proliferate on the media used in subculturing. After repeated proliferation, these tissues could be induced to form mature embryos in 1 - 3 months using MS2 embryogenic tissue induction media. Torpedo embryos and cotyledon embryos could not directly proliferate on MS3 media, but highly embryogenic calli could be induced from them using embryogenic callus induction media (MS4). The highly embryogenic calli could further be induced to form a large number of embryos on MS5 media to achieve an indirect but efficient proliferation. By selecting the same physiological stage of embryonic tissue from the material to be subcultured, the process of somatic embryo development could be controlled to ensure uniform development.

K1 Heveabrasiliensis, embryogenic tissue, somatic embryo, anther culture PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-320