RT Journal T1 CHARACTERIZATION OF ENDO-β-MANNANASE ACTIVITY AND GENE EXPRESSION DURING ‘YANHONG’ PEACH STORAGE A1 Y. F. Ren A1 J. Y. He A1 L. L. Zhang JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 25 IS 3 SP 122 OP 127 YR 2015 FD 2015/06/01 DO DOI NA AB

Fruit softening is mainly caused by the cell wall modifications. Mannan is one of cell wall components, and its complete breakdown requires endo-β-mannanase (EC 3.2.1.78, β-Man). In the present study, the expression pattern of β-Man activities and genes in fruits, as well as the changes of fruit firmness and ethylene production were monitored during peach fruit storage. The results showed that the β-Man activity in both skin and mesocarp increased concomitantly with the loss of firmness and ethylene release during peach fruit storage. In addition, β-Man activity in skin was significantly higher than that in mesocarp. Two partial cDNAs of PpMAN1and PpMAN2 were obtained from mature peach fruit. The accumulation of PpMAN1 and PpMAN2 in both tissues exhibited a steady decline with the peach fruit softening during storage, which positively correlated with the loss of fruit firmness, but negatively correlated with the increase of β-Man activity and ethylene production.

K1 Endo-β-mannanase, peach fruit, activity, gene expression, softening PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-318