RT Journal T1 EFFECT OF 5-AMINOLEVULINIC ACID AND GENISTEIN ON ACCUMULATION OF POLYPHENOL AND ANTHOCYANIN IN ‘QINYANG’ APPLES A1 L. Chen A1 Y. R. Guo A1 G. Bai A1 J. J. Sun A1 Y. Li JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 25 IS 3 SP 68 OP 79 YR 2015 FD 2015/06/01 DO DOI NA AB

Consumers are highly interested in purchasing apples with red color. However, the early-ripening apples always show poor color, so it is particularly important to improve apple color. The study was aimed to investigate effects of 5- Aminolevulinic acid (ALA) and/or Genistein (GNT) on apple peel color of ‘Qinyang’, polyphenol compounds and regulation of the gene related to anthocyanin synthesis. 300 mg·L-1 ALA and/or 21.62μg·L-1 GNT were sprayed on ‘Qinyang’ apples every five days after their bags were removed. The concentrations of anthocyanin and total polyphenol were determined with spectrophotometer and the concentrations of anthocyanin and polyphenol components were determined by HPLC. The relative expressions of six anthocyanin-related structural genes and regulation factor MYB10 were measured by RT-PCR. Spraying ALA and/or GNT had significant promoting effects on anthocyanin accumulation of apple peel. The contents of anthocyanin with ALA, GNT, and ALA plus GNT sprayed were 1.12, 1.37, and 3.00 times as much as that with water sprayed, respectively. ALA and/or GNT played an important role in promoting total polyphenol accumulation, and the flavonoids concentrations of apple peel were significantly increased. The RT-PCR results revealed that ALA and/or GNT up-regulated the expressions of PAL, CHS, DFR, F3H, UFGT, LDOX and MYB10.

K1 Apple, 5-Aminolevulinic acid, Genistein, Anthocyanin, Polyphenol, Gene expression PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-311