RT Journal T1 MOLECULAR CHARACTERIZATION OF THE σC-ENCODING GENE FROM NOVEL DUCK REOVIRUS A1 T. Yun A1 H. P. Chen A1 J. G. Hua A1 B. Yu A1 L. Chen A1 Z. Ni A1 W. C. Ye A1 C. Zhang JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 25 IS 3 SP 33 OP 41 YR 2015 FD 2015/06/01 DO DOI NA AB

We cloned and sequenced the σC-encoding gene of a novel duck reovirus (N-DRV) isolate ZJ00M. The σC gene is 966 bp long and is encoded by the third ORF of the S1 segment, a small protein of 321 amino acid residues (approximately 34.1 kDa). Expressed σC fusion protein in E. coli showed good antigenicity and immunogenicity in a western blotting assay and indirect immunofluorescence assays (IFA). The comparative sequence analyses indicated that N-DRV σC shared high identity levels with ARV species (classical MDRV and ARV) and a lower degree of identity with other orthoreovirus counterparts, but the sequence identities were no more than 45 %. As observed in classical MDRV and ARV σC protein sequences, some sequence motifs, conserved sequence motifs and/or functional domains were identified in the sequence of N-DRV σC. The phylogenetic analysis of σC/σ1 sequence revealed that N-DRV was different fromother orthoreovirus species groups but remained classified into the avian orthoreoviruses along with other ARVs, suggesting that N-DRV and classical MDRVs constitute a separate geno group relative to ARV. These results are important for future studies examining the structure, function and immunogenicity of N-DRV σC.

K1 Novel duck reovirus, σC-encoding gene, Expression, Phylogenetic analysis, Genotype PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-306