RT Journal
T1 BIO-PHYSIOLOGICAL EFFECTS OF LD50 OF CRUDE VENOM OF BLACK PAKISTANI COBRA (Naja Naja karachiensis) IN MICE
A1 Z. Riaz
A1 M. Q. Zaman
A1 Z. Ullah
A1 Habib-ur-Rehman
A1 M. S. Yousaf
A1 I. Rabbani
A1 M. I. Khan
A1 M. S. Hussain
A1 S. Tahir
A1 K. A. Majeed
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 25
IS 5
SP 1344
OP 1348
YR 2015
FD 2015/10/01
DO DOI NA
AB <p>The present study evaluated the acute bio-physiological effects of Lethal dose 50 (LD 50) of crude venom of Black Pakistani Cobra,&nbsp;<em>Naja naja karachiensis</em>, collected from Southern Punjab, Pakistan. Adult mice (n=20) were injected intramuscularly with normal saline (n/s) or LD50 of crude venom (1.2mg/kg). Blood was collected by cardiac puncture after anesthesia at intervals 0 (n/s), and 1, 1.5, 2 (LD50 venom) hours. Complete blood count, glucose, serum Alanine aminotransferase (ALT), albumin, total proteins and urea were measured to assess the physiology. One way ANOVA was used to analyze the data (P&lt;0.05). A significant increase in erythrocyte count, its indices and thrombocytosis was observed during the study. Leukocyotsis was observed after a transient leucopenia at 1 hour post envenomation. No significant difference was observed for lymphocytes and monocytes. Serum concentrations of urea, total proteins, albumin and ALT were also increased significantly with all time intervals. Hypoglycemia was observed initially followed by hyperglycemia at 1.5 and 2 hours. The changes indicate that venom has severe hemotoxic potential and disrupts physiological processes by affecting vital organs like kidneys and liver in mice.</p>
K1 Cobra venom, Hematology, Bio-physiological effects, Lethal dose 50, Mice
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-183