RT Journal T1 INFLUENCE OF ZHUQIN FORMULA EXTRACT ON THE CELLULAR AND HUMORAL IMMUNE RESPONSE IN CYCLOPHOSPHAMIDE-IMMUNOCOMPROMISED MICE A1 L. Juan A1 Q. Hong A1 Z. Z. Rong A1 Z. M. Xun A1 L. Y. Chen A1 Y. Z. Qiang A1 L.G. Shu A1 Y. L. Hua A1 Y. Qiang JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 25 IS 5 SP 1335 OP 1343 YR 2015 FD 2015/10/01 DO DOI NA AB

The aim of this study was to evaluate efficacy of extract from traditional Chinese medicine Zhuqin formula (ZQE) in cyclophosphamide-immunosuppressed mice. A total of 340 four weeks old Kunming mice were taken for the study. The study is divided into two parts. One part is to determine a safe dose of ZQE by gastric perfusion in mice with an acute toxicity and a maximum tolerance test, and the second part of the presented study indicates the immunorestorative effects of ZQE dependent of the doses applied in mice treated with cyclophosphamide, including the carbon clearance index, the weight ratio of immune organ, serum hemolysin, T-, B-lymphocyte transformation rate and IL-2, IFN-γ, and IL-4 secretion. The acute toxicity test indicated that LD50 of ZQE by gastric perfusion in mice was > 5,000 mg/kg.Themaximum tolerated dose of ZQE (oral administration) was > 120 g/kg. In the treating immunosuppressed mice test, three doses of ZQE groups could increase the carbon clearance index, the weight ratios of spleen and thymus, serum hemolysin level, promote the secretion of IL-2, IL-4, and IFN-γ, and T-, B-lymphocyte transformation rate by 1.81-, 1.73-, 2.67-, 2.36-, 1.96-, and 2.69-fold of model group,respectively, and a 30mg/kg dose of ZQE presented a significantly better outcome, compared with the 40 and 20 mg/kg doses.

K1 cyclophosphamide; immunosuppression; spleen; thymus; cytokine; mice PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-182