RT Journal
T1 EVALUATION OF DNA EXTRACTION METHOD FROM FORMALIN PRESERVED SKIN SAMPLES OF PANTHERA PARDUS FOR MOLECULAR GENETIC ASSESSMENT
A1 S. S. Bibi
A1 A. Rehman
A1 R. A. Minhas
A1 S. Janjua
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 25
IS 4
SP 1196
OP 1199
YR 2015
FD 2015/08/01
DO DOI NA
AB <p>Five different protocols,<em>&nbsp;viz.&nbsp;</em>Andleeb&nbsp;<em>et al.</em>&nbsp;(2012), Shrivastava&nbsp;<em>et al</em>. (2012), Phenol chloroform DNA extraction method, Roboscreen Kit method and Phenol chloroform triton method, were tested for DNA extraction from skin samples of Leopard (<em>Panthera pardus)&nbsp;</em>preserved in formalin over a period of two to five months. Among these procedures, the Phenol chloroform triton method was proved to be the most efficient protocol with 83% extraction rate and revealed 100% amplification of FCA026 and FCA043 loci. Keeping in views the extraction and amplification efficiency, Phenol chloroform triton method is recommended for molecular studies of formalin preserved skin samples of&nbsp;<em>Panthera pardus</em>.</p>
K1 Skin Tissue Samples, DNA Extraction, Polymerase Chain Reaction
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-161