RT Journal T1 DEVELOPMENT OF STS MARKERS FOR WAXY GENE ALLELIC VARIATION IDENTIFICATION IN FOXTAIL MILLET A1 X. P. Jia A1 P. H. Dong A1 F. Y. Yu A1 J. Z. Quan A1 H. Bai A1 Z. P. Dong JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 25 IS 4 SP 1191 OP 1195 YR 2015 FD 2015/08/01 DO DOI NA AB

Waxy gene controls synthesis of amylose, having important application value in crop eating quality improvement. The waxy gene of foxtail millet has been cloned and sequenced. All the waxy and low-amylose types arose by the insertions of transposable elements into the waxy gene. Based on the sequence variation information caused by transposon insertions, 6 pairs of STS primers were designed to detect 10 allelic variation types of the waxy gene in eleven foxtail millet strains. The results showed that waxy allelic variation types of nine strains could be determined, they were “Xinji” sticky millet (IV/IV), Green sticky millet (IVa/ IVa), “Shanxi” sticky millet (VII/Wild), “Anyanghong” sticky millet (IV/IV), Jite5 (IV/III), Jigu30 (IV/V), Yellow sticky millet (IVa/ IVa), “Linxian” black sticky millet (IV/ VII), “Xincai” sticky millet (VII/ Wild). Two modern varieties Jigu19 and “Early white waxy” detected three allelic variation types (IVa, III, V), the real reason need be further investigated. No X, VI, VIII variation types were detected in the eleven foxtail millet strains. The developed STS markers were powerful in detecting which of the 10 waxy allelic variation types existed in any foxtail millet strain though iodine blue staining amylose determination technology needed when distinguish homozygous variation types from heterozygotes of variation type and wild type because of dominant markers.

K1 Foxtail millet, Eating quality, Waxy gene, Allelic variation, STS marker PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-160