RT Journal
T1 CHARACTERIZATION OF MYCOPLASMA GALLISEPTICUM ISOLATED FROM COMMERCIAL POULTRY FLOCKS
A1 M. A. Gondal
A1 M. Rabbani
A1 K. Muhammad
A1 T. Yaqub
A1 M. E. Babar
A1 A. A. Sheikh
A1 A. Ahmad
A1 M. Z. Shabbir
A1 M. I. Khan
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 25
IS 1
SP 108
OP 113
YR 2015
FD 2015/02/01
DO DOI NA
AB <p>Chronic Respiratory Disease (CRD) is caused by&nbsp;<em>Mycoplasmas gallisepticum</em> (MG) and is prevailing in layer, broiler and breeder poultry flocks. The infected birds showed sneezing, rales, coughing and exudates from nostrils and eyes. Mycoplasma species were recovered 27.3% overall, 39.3 per cent of the tracheal swabs, 15.9 per cent of tracheal tissues, 27.4 per cent of lung tissues and 25 per cent of air sacs. Polymerase chain reaction confirmed the nucleic acid of the MG from tracheal swabs (68.18%), tracheal tissues (42.47%), lung tissues (31.85%) and air sacs (50%) using universal and MG specific primer. Higher percentage of MG confirmation through PCR is due to detection of nucleic acid of MG from medicated birds. It is concluded that culture method was laborious and time consuming and failed to detect mycoplasma species from medicated birds and is less sensitive than that of PCR. PCR is rapid, sensitive and accurate method for diagnosis of MG from suspected cases.</p>
K1 Mycoplasma gallisepticum, CRD, PCR, Frey’s broth
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-16