RT Journal
T1 POTATO VIRUS X FROM PAKISTAN: COAT PROTEIN SEQUENCE ANALYSIS
A1 A. Khan
A1 B. Tabassum
A1 I. A. Nasir
A1 M. Bilal
A1 M. Tariq
A1 T. Husnain
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 25
IS 4
SP 1016
OP 1021
YR 2015
FD 2015/08/01
DO DOI NA
AB <p>Potato virus X (PVX) a most devastating pathogen of potato, tomato and tobacco all over the world was surveyed for its epidemics in Pakistan. A 891bp amplicon covering full capsid protein gene of potato virus X was amplified through reverse transcription-polymerase chain reaction by using gene specific primers from the samples collected all over Punjab, Pakistan. Deduced sequence of full capsid protein gene of PVX was reported as the first full length coat protein gene of potato virus X from Pakistan in NCBI database (Accession # KC757709). In phylogenetic analysis, Pakistani PVX isolate was found to be clustered closely with European sub linage while distinctly with Australian PVX and South American isolates. In contrast to the nucleotide sequence the amino acid sequence dendrogram of PVX, Pakistani isolate showed 100% homology with American isolates. Conclusively, a new isolate of PVX was identified from Pakistan which surprisingly exhibited close lineage with European isolate.</p>
K1 Potato Virus X, Capsid protein gene, Phylogenetic analysis, Reverse Transcription, amino acid sequence dendrogram
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-136