RT Journal
T1 PLASMID PROFILING AND CURING OF MULTIDRUG RESISTANT ESCHERICHIA COLI RECOVERED FROM RETAIL CHICKEN MEAT
A1 M. Gohar
A1 A. A. Sheikh
A1 A. A. Anjum
A1 T. Hussain
A1 J. Muhammad
A1 A. Tabbassum
A1 A. Kanwal
A1 I. Safdar
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 25
IS 4
SP 984
OP 988
YR 2015
FD 2015/08/01
DO DOI NA
AB <p>Confirmed<em>&nbsp;E. coli&nbsp;</em>(n = 25) were analyzed for the presence of plasmids and cured using temperature and sodium dodecyl sulphate (SDS) treatments. Plasmid profiling of the isolates showed that 96 % isolates were harboring plasmids of varying size and number. The average plasmid number among the isolates was 2.28, ranging from 0 &ndash; 5 plasmids per isolate and plasmid size varied from 100bp &ndash; 12kb which was distributed randomly in these isolates. Statistically no significant correlation (<em>p</em>&gt;0.05) was found between antibiotic resistance and plasmid profiles. Plasmid curing study showed 3.9% and 16.7% curing efficiency with that of temperature and SDS treatment, respectively.<strong>&nbsp;</strong>Statistical analysis revealed that resistant isolates, treated with either physical or chemical treatments produced significant results (<em>p</em>&lt;0.05) of plasmid curing. Antimicrobial resistance genes might be located on plasmid and statistically no relationship was observed between antibiotic resistance and plasmid profiles.</p>
K1 Plasmid profiles, plasmid curing, E. coli, antibiotic resistance
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-131