RT Journal T1 HETEROLOGOUS EXPRESSION OF MSHSP23, A MEDICAGO SATIVA SMALL HEAT SHOCK PROTEIN, ENHANCES HEAT STRESS TOLERANCE IN CREEPING BENTGRASS A1 K. W. Lee A1 J. Y. Cha A1 J. Y. Mun A1 B. H. Lee A1 Y. G. Kim A1 S.H. Lee JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 25 IS 3 SP 884 OP 891 YR 2015 FD 2015/06/01 DO DOI NA AB

Small heat shock proteins (Hsps) are conserved in living organisms. They exist in diverse subcellular organelles and play important roles in plant defense systems against various abiotic stresses. Chaperone properties of small Hsps have been widely known to prevent the stress-induced denaturation of substrate proteins in cells. Here, we examined the capacity ofMsHsp23to confer tolerance to heat stress (thermotolerance)by heterologous expression in creeping bentgrass. We generated nine independent transgenic creeping bentgrass plants. Two independent transgenic plants (Tg-1 and Tg-2) were examined forthermotolerance (42°C/24h).This resulted in minimal wilting ofthe leaves, which retained healthy green color, while the non-transformed (NT) plants wilted and showed light-green color of their leaves. Plants overexpressing MsHsp23 displayed higher ascorbate peroxidase (APX) activities. Therefore, we conclude that heterologous expression of MsHsp23 in creeping bentgrass can protect plants against heat stress, presumably bychaperone activity that allows for induction of APX.

K1 Small Heat-shock protein, Thermotolerance, Creeping bentgrass, Antioxidant enzymes PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2015-JAPS-119