RT Journal T1 TERATOGENIC AND EMBRYO-TOXIC EFFECTS OF LATEX AND LEAVES METHANOL EXTRACT OF EUPHORBIA HELIOSCOPIA L. IN MICE AND CHICKEN EMBRYOS A1 U. Saleem A1 B. Ahmad A1 M. Ahmad A1 K. Hussain A1 N. I. Bukhari A1 A. A. Anjum JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 24 IS 2 SP 450 OP 454 YR 2014 FD 2014/04/01 DO DOI NA AB

Euphorbia helioscopia L. has been investigated for antibacterial, antifungal, antiviral and vasodepressor activities. The study was designed to probe into the reproductive toxicity (teratogenicity) potential of Euphorbia helioscopia L. in albino mice. Toxicity of latex and methanol extract of leaves (10, 50, 80, 100 and 120 µg/200 µL) was evaluated in white leghorn chicken embryos (5th day) using chorioallantoic membrane route (n = 30 for each experiment i.e. latex & extract, or 5 per dose and 5 as control group). Teratogenic potential of latex (n= 5) and extract (100, 300, 500, 700, 1000, 1500 and 2000 mg/kg body weight, n= 40, or 5 per concentration and 5 as control group) was evaluated in pregnant albino mice on 7th day of gestation using intra-peritoneal route. Both latex and leave extract checked in chicken embryos were safe. LD50 calculated with TSK software was 1397 mg/kg body weight for methanol extract of leaves observed in albino mice. The rest of the experimental concentrations were non-lethal in mice without any observable malformation. It is concluded that the concentration (administered parentally) less than LD50 is devoid of teratogenic effects in pregnant female mice and chicken embryos.

K1 Euphorbia helioscopia, Chicken Embryo, Mice, Teratogenicity, Toxicity PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-64