RT Journal T1 A PROTEOMIC BASED ASSESSMENT ON CHANGES IN MYOFIBRILLAR PROTEINS OF GOAT LONGISSIMUS MUSCLE AS AFFECTED BY HEAT TREATMENTS A1 S. A. Saraha A1 S. A. Karsanic A1 d A1 I. Amina A1 N. F. K. Mokhtara A1 A. Q. Sazilib JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 24 IS 2 SP 406 OP 412 YR 2014 FD 2014/04/01 DO DOI NA AB

The present study examined the effect of different heat treatments; (1) chilled, (2) boiled at 100°C for 30 min, and (3) autoclaved at 121°C at 15 psi for 20 min, on the expression of goat skeletal muscle proteins using two-dimensional gel electrophoresis. The molecular weight (MW) and isoelectric point (pI) of heat stable proteins were characterised followed by identification of the proteins by MALDI-TOF/TOF mass spectrometry. There were 153 protein spots obtained in the boiled samples, while only 46 protein spots were observed in the autoclaved samples. Thirteen spots that exhibited high intensity of protein were chosen from the autoclaved sample for MALDI-TOF/TOF mass spectrometry analysis. The putative heat stable proteins identified were myosin light chain (MLC), actin, tropomyosin (TPM), troponin T (TnT), myoglobin, and creatine kinase. The Proc-GLM analysis revealed that the heat treatments have resulted in significant differences in spot intensities of actin, troponin T (TnT), myoglobin, and creatine kinase with no significant changes noted in other proteins. 

K1 goat meat, heat treatments, myofibrillar protein, 2-dimensional electrophoresis, MALDI-TOF/TOF PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-57