RT Journal
T1 MOLECULAR CHARACTERIZATION AND PHYLOGENETIC RELATIONSHIP OF DIFFERENT CITRUS VARIETIES OF PAKISTAN
A1 S. Naz
A1 K. Shahzadi
A1 S. Rashid
A1 F. Saleem
A1 A. Zafarullah
A1 Shabbir Ahmad
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 24
IS 1
SP 315
OP 320
YR 2014
FD 2014/02/01
DO DOI NA
AB <p>Genetic relationship among 17 citrus commercially important cultivars, including local and some hybrid varieties of Pakistan was evaluated by RAPD (Random Amplified Polymorphic DNA) markers. Fifteen decamer primers were selected out of 25 primers that produced 153 polymorphic bands with 13bands per primer on an average with a total of 188 bands. To analyze the genetic similarity or dissimilarity Jaccard Coefficients was used. The dendrogram that separated 17 citrus genotypes into 4 main clusters was generated by using UPGMA. Across all the genotypes the genetic similarity value observed were 0.66 with the two cultivars of Kinnow and Seedless kinnow (Mandarin) and two cultivars of Meiwa and Marumi (Kumquat) showing maximum similarity value (0.85).Genetic difference among the citrus varieties was high that depicted that they do not belong to similar origin.</p>
K1 Genetic diversity, Citrus varieties, RAPD, Polymerase chain reaction, phylogenetic relationship
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-44