RT Journal T1 MICROARRAY GENE EXPRESSION MIAME-PLANT SUPPORTIVE TAB DELIMITED DATA FORMAT: MAGE-TAB-PLANT A1 S. U. Rehman A1 Atif A. Mirza A1 K. Masood A1 B. Rashid A1 T. Husnain JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 24 IS 1 SP 179 OP 189 YR 2014 FD 2014/02/01 DO DOI NA AB

The goal of Minimum Information about a Microarray Experiment (MIAME) is to outline the minimum information that can clearly state the microarray based gene expression experiment in such a way that the details of experiment design, description and sample annotation can help to check and reproduce the results in some other laboratory. MIAME/plant complements the MIAME standard with guideline for experiment design, description and sample preparation protocols using controlled vocabularies. MAGE-TAB-Plant format has been structured on the similar lines as MAGE-TAB format.  It explains the main chain of all events in a typical Microarray based experiment such as steps involved in preparation and handling of samples, labelling and hybridization protocols, scanning of images, normalization of raw data and analysis of images. The physical entities in this chain are Biosource, Sample, Extract, labelled Extract, hybridization, scan image, raw, normalized and combined data matrix. It is hoped that little more effort may result in better precisely replicated, analyzed, and better interpreted reproducible plant based microarray experiments. Data mining capabilities of data submitted using MAGE-TAB-Plant format may generate breakthroughs in research and the use of controlled vocabularies may encourage efficient sharing and annotation of the experiment data.

K1 Microarray, Data format, Tab delimited, MIAME PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-27