RT Journal T1 ISOLATION AND CHARACTERISATION OF COLLAGEN FROM THE WASTE MATERIAL OF TWO IMPORTNT FRESHWATER FISH SPECIES A1 S. Mahboob A1 S. Haider A1 S. Sultana A1 K. A. Al- Ghanim A1 F. Al-Misned A1 H. F. Al-Kahem Al-Balawi A1 Z. Ahmad JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 24 IS 6 SP 1802 OP 1810 YR 2014 FD 2014/12/01 DO DOI NA AB

The study was designed to isolate and characterize the collagen from skin, scales and fins of Cyprinus carpio and Hypophthalmichthys molitrix. For this purpose nine fishes of each species of three weight groups were collected and categorized as W1 (250-500 g), W2 (501-750 g) and W3 (751- 1000 g). The maximum skin protein content was recorded as 28.13% and 29.13% in C. carpio and H. molitrix, respectively, from the W3. The scales of the W3 displayed a maximum protein contents as 29.91 and 28.38% for C. carpio and H. molitrix, respectively. The maximum protein contents (19.19% and 21.37% for C. carpio and H. molitrix, respectively) were observed in the W3 group. Protein characterisation using SDS-PAGE revealed the molecular weights (kDa) of the C. carpio skin, scales, and fins ranged from 126 kDa to 220 kDa, 78 kDa to 211 kDa, and 72 kDa to 141 kDa, respectively. The size of the collagen proteins of the H. molitrix skin, scales and fins ranged from 117 kDa to 208 kDa, 81 kDa to 219 kDa, and 72 kDa to 141 kDa, respectively. Glycine was the most abundant amino acid, whereas, Tryptophan was totally absent in all selected tissues. Significant differences were observed for amino acids within the weight groups and between the two fish species.

K1 Fish; Proximate composition; weight groups; Amino acids; SDS-PAGE PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-258