RT Journal T1 IN VITRO PLANT REGENERATION OF TURKISH SWEET BASİL (OCIMUM BASILICUM L.) A1 H. Ekmekci A1 M. Aasim JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 24 IS 6 SP 1758 OP 1765 YR 2014 FD 2014/12/01 DO DOI NA AB

Sweet Basil (Ocimum basilicum L.) is most important medicinal and aromatic plant used in aromatherapy, cosmetics, perfume and food products in fresh or dried form. The present study provides a reliable and reaptable in vitro plant regeneration protocol of cultivated sweet basil of Turkey. Basil seeds were surface sterilized with 2.5% NaOCl. Epicotyl, hypocotyl and shoot tip explants were isolated from 12-14 days old in vitro grown seedlings. The explants were cultured on MS medium containing 0.80-2.40 mg/L TDZ with or without 0.10 mg/L IBA alongwith 1.0 mg/L PVP and 3.0 g/L activated charcoal. Cent percent callus induction were recorded on all explants on all culture mediums. Shoot regeneration frequency of epicotyl, hypocotyl and shoot tip explant ranged 75.0-100, 25.0-83.33 and 66.67-100% respectively. Maximum number of shoots from epicotyl (3.22) and shoot tip (3.58) were scored on MS medium containing 2.40 mg/l TDZ-0.10 mg/l IBA. Whereas, hypocotyl explant induced maximum number of (5.17) shoots per explant on MS medium with 2.0 mg/l TDZ. Relatively shorter roots of less than 1.0 cm were recorded irrespective of explant type or culture medium. However, shoots were successfully rooted and plantlets were transferred to pots and acclimatized well in growth room conditions.

K1 In vitro, Epicotyl, Hypocotyl, Shoot tip, Shoot regeneration, Sweet basil PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-252