RT Journal
T1 EXPERIMENTAL STUDY ON TISSUE TROPISM AND DISSEMINATION OF H9N2 AVIAN INFLUENZA VIRUS AND ORNITHOBACTERIUM RHINOTRACHEALE CO-INFECTION IN SPF CHICKENS
A1 A. Azizpour
A1 H. Goudarzi
A1 S. Charkhkar
A1 R. Momayez
A1 M. H. Hablolvarid
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 24
IS 6
SP 1655
OP 1662
YR 2014
FD 2014/12/01
DO DOI NA
AB <p>Forty-two, one<span dir="rtl">&nbsp;</span><span dir="rtl">-</span>day<span dir="rtl">&nbsp;</span><span dir="rtl">-</span>old specific pathogen-free (SPF) chickens were randomly divided into two groups.&nbsp; On 21st day of study, experimental group was infected intraocularly with 1&times;106 EID50 of H9N2 and intratracheally with 1<span dir="rtl">&nbsp;</span><span dir="rtl">&times;</span><span dir="ltr">&nbsp;</span>1010 CFU of Ornithobacterium rhinotracheale (ORT). Control group was inoculated with sterile PBS intraocularly. The samples from various tissues were collected on<strong>&nbsp;</strong>days 2, 4, 6, 8, 10, 12, 14 and 16<strong>&nbsp;</strong>post-inoculation (PI). Experimental group chickens exhibited more severe respiratory signs, depression, anorexia and 15% mortality. The ORT was isolated in the trachea from days 2&ndash;4 PI and in the lungs on day 4 PI. The H9N2 virus was detected in the lungs and trachea from days 2&ndash;4 PI. The virus was also detected in the bursa of fabricius on days 2 and 6 PI and in thymus and liver on day 2 PI. The virus was found only on day 8 PI in kidneys. The virus detection from organs was reduced with increasing antibody titer on day 8 PI. The results of this study indicates that concurrent infection with H9N2 virus and ORT can exacerbate virulence and lesions of H9N2.</p>
K1 H9N2 influenza virus, Ornithobacterium rhinotracheale, Co-infection, Tissue tropism, SPF chickens
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-237