RT Journal
T1 SEQUENCE-RELATED AMPLIFIED POLYMORPHISM (SRAP) FOR STUDYING GENETIC DIVERSITY AND POPULATION STRUCTURE OF PLANTS AND OTHER LIVING ORGANISMS: A PROTOCOL
A1 X. Y. Li1
A1 J. Li
A1 Z. J. Zhao
A1 F. Yang
A1 Q. W. Fu
A1 H. S. Liu
A1 D. D. Wang
A1 Y. C. Yang
A1 R. Y. Wang
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 24
IS 5
SP 1478
OP 1486
YR 2014
FD 2014/10/01
DO DOI NA
AB <p>Genetic variation is widespread in living organisms. The accurate analysis of genetic variation and studies of population structures have significant implications for understanding the genetic traits, population genetics, molecular breeding and disease control programs in plants and animals. Sequence-related amplified polymorphism (SRAP) analysis has been widely used for studying genetic diversity and population structures of living organisms in the last decade. This molecular approach preferentially targets the open reading frame (ORF) regions of the genome with universal primers to screen the mutations across the whole genome, allowing to investigate genetic variations in the genome, and to develop new specific markers for species or population differentiation and molecular breeding. This article briefly outlined the applications of SRAP technique in plants and animals, and detailed described the protocol of this technique. It will make the usage of this approach conveniently and maneuverably, and further expand its applications to a wider range of living organisms.</p>
K1 Sequence-Related Amplified Polymorphism (SRAP), Protocol Review, Genetic Diversity, Population Structure
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-211