RT Journal
T1 ASSESSMENT OF GENETIC VARIABILITY AMONG SELECTED SPECIES OF APOCYNACEAE ON THE BASIS OF rps 11 GENE
A1 M. Ibrahim
A1 N. Nazar
A1 M. Ilyas
A1 T. Mahmood
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 24
IS 4
SP 1266
OP 1269
YR 2014
FD 2014/08/01
DO DOI NA
AB <p>The importance of genetic variation for sustaining biological diversity and evolutionary processes is widely accepted. The present study aims to investigate the phylogenetic relationships among five selected species of Apocynaceae collected from various regions of Pakistan using chloroplast gene (<em>rps</em>11) which encode ribosomal protein of smaller subunit11. For this purpose&nbsp;<em>rps</em>11 gene was amplified, purified, sequenced from each species and analyzed with MEGA5. Phylogenetic analysis revealed an overall narrow genetic background (0.5 %) among selected species.&nbsp;<em>Nerium oleander</em>,&nbsp;<em>Rhazya stricta</em>&nbsp;and&nbsp;<em>Vinca major</em>&nbsp;were the most similar with a bootstrap value of 24-44 % while&nbsp;<em>Catharanthus roseus</em>&nbsp;and&nbsp;<em>Nerium oleander</em>&nbsp;showed distant relationship. Divergence value was observed within a range of 0.023-9.994 on the basis of pair wise distance calculation. The study further confirmed the use of&nbsp;<em>rps</em>11 gene as a potential candidate for phylogenetic analysis.</p>
K1 Apocynaceae, rps11, Genetic Variation
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-183