RT Journal T1 EXTRACELLULAR PHYTASE PRODUCTION BY BACILLUS SP. T4 USING SOLID STATE FERMENTATION A1 J. Lee A1 I. Park1and J. Cho JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 24 IS 4 SP 1116 OP 1122 YR 2014 FD 2014/08/01 DO DOI NA AB

Wheat bran, soybean meal, corn flour and the combinations of these individual substrates with nutritive supplements containing 1% casein hydrolysate, 0.2% CaCl22H2O, 0.4% (NH4)2SO4, 0.02% MgSO47H2O, 0.05% KH2PO4 and0.04% K2HPO4 were evaluated to select an optimal medium in solid state fermentation (SSF) to produce extracellular phytase from a bacterial strain, Bacillus sp. T4. The combination of corn flour with nutritive supplements resulted in best phytase synthesis and was used for further SSF explorations. Maximum phytase production (20787±39 U/g) was observed at a growth period of 84 h, 55.5% moisture content, and 4% inoculum density. Optimum pH for phytase production was 7.0. Enzyme activity was enhanced (P<0.05, 16496±187 to 18304±187 U/g) in the presence of glucose and galactose as a carbon source. The additional nitrogen sources impaired (P<0.05) the phytase activity, and corn steep liquor and sodium nitrateseverely inhibited the enzyme synthesis. T4 phytase produced in SSF may be a promising strategy for upgrading the nutritional quality and combating environment pollution in the feed industry.

K1 Solid state fermentation, phytase, Bacillus sp., feed industry PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-161