RT Journal
T1 NANO-GOLD PARTICLES MEDIATED DETECTION OF NS1; AN EARLY DIAGNOSTIC MARKER OF DENGUE VIRUS INFECTION
A1 A. Hussain
A1 S. U. Rehman
A1 S. Aslam
A1 N. Javed
A1 Z. Abbas
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 24
IS 4
SP 1110
OP 1115
YR 2014
FD 2014/08/01
DO DOI NA
AB <p>In this study, an immunoassay is developed for qualitative determination of NS1 antigen of dengue virus in serum using nano-gold particles. This diagnostic device comprises of four components such as sample pad, conjugation pad, nitrocellulose membrane and absorbent pad. Anti-NS1 antibodies and rabbit anti-mouse IgG antibodies were coated on nitrocellulose membrane as a test and control line respectively. Assay device was evaluated by purified NS1 and prior ELISA confirmed NS1 positive and negative samples. The diagnostic device showed promising results to detect NS1.</p>
K1 Dengue Virus, Diagnostic Device, Colloidal Gold Particles, Rapid Diagnosis, NS1 antigen, ELISA
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-160