RT Journal
T1 ISOLATION AND MOLECULAR DETECTION OF CLOSTRIDIUM CHAUVOEI ALPHA TOXIN GENE FROM CLINICAL CASES OF BLACK QUARTER IN CATTLE
A1 A. Idrees
A1 Z. I. Chaudhary
A1 M. Younus
A1 K. Ashraf
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 24
IS 3
SP 755
OP 759
YR 2014
FD 2014/06/01
DO DOI NA
AB <p>The objective of the present study was to isolate and identify&nbsp;<em>Clostridium chauvoei</em>&nbsp;from swab (n=750) and tissue (n=50) samples from cattle suspected for black quarter in six districts belonging to different climatic zones (Punjab, Pakistan). Conventional culture methods showed 192(25.6%) and 19(38%) positive from swab and tissues respectively for this bacterium. These bacterial isolates were further processed and confirmed by Polymerase chain reaction (PCR) by amplifying and (Ccta) gene sequences. An amplicon of 836 bp corresponding to 16SrRNA genesequence was obtained. PCR confirmed 140 (72.9%) and 41 (82%) positive samples from swab and tissues respectively.It is concluded that PCR was found an efficient tool for precise detection of&nbsp;<em>Clostridium chauvoei</em></p>
K1 Black quarter, Clostridium chauvoei, cattle, PCR
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2014-JAPS-106