RT Journal T1 DIFFERENTIAL EXPRESSION AND BIOINFORMATICS ANALYSIS OF MIR-184 IN PREGNANT AND NON-PREGNANT GOAT OVARIES A1 X. Zhang A1 J. Ding A1 Y. Ling A1 Y. Li A1 Y. Zhang A1 X. Zhang JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 23 IS 6 SP 1545 OP 1552 YR 2013 FD 2013/12/01 DO DOI NA AB

Ovarian follicular development and hormone secretion are complex and coordinated biological processes which alter during pregnancy. Ovarian function is tightly regulated by a multitude of genes, and may also be regulated by specific miRNAs. It is necessary to identify the differentially expressed miRNAs in the ovaries of pregnant and non-pregnant mammals, in order to fully understand the role of miRNA-mediated post-transcriptional regulation in mammalian reproduction. In an effort to uncover miR-184 importance in goats, we examined the differential expression pattern in the ovaries of 5 pregnant and 5 non-pregnant goats and gene ontology (GO) term enrichment of predicted target genes to determine the global biological functions of miR-184. Quantitative real-time PCR (q-PCR) analysis showed that miR-184 was expressed in the ovaries of pregnant goats, but was not expressed in the ovaries of non-pregnant goats. Moreover, thirty-eight target genes of miR-184 were predicted and 10 functional categories of GO terms were significantly overrepresented among the predicted miR-184 target genes. These categories can be broadly defined as regulation of development process and cellular biosynthetic process. Our results demonstrated that miR-184 was involved in various biological processes including pregnancy, developmental and cellular biosynthetic processes. Stephens and Moley 2009) . In the non-pregnant phase,

K1 miR-184 expression, GO term enrichment, ovaries, pregnant goat PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2013-JAPS-611