RT Journal T1 MORPHOLOGICAL AND MORPHOMETRICAL STUDIES OF TRYPANOSOMA DANILEWSKYI STRAIN FCC-1. A1 M. S. Ahmed A1 K. Shafiq H. Ali A1 F. Ollevier JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 23 IS 4 SP 1041 OP 1044 YR 2013 FD 2013/08/01 DO DOI NA AB

Morphological and morphometrical study was conducted on blood stream forms of Trypanosoma danilewskyi strain F Cc-1, isolated from laboratory-infected single breed juvenile common carp. A group of ten fish was intraperitoneally inoculated with 1000 live trypanosomes per fish. At the peak of parasitemia thin blood smears were prepared on precleaned glass slides on days 20, 30, 40 and 50 post infections (p.i). Blood smear was air dried, fixed with methanol and stained with May-Grünwald Giemsa stain. Stained trypanosomes were examined under light microscope at X 1000 magnification in the immersion oil. Morphology of trypanosome was recorded with a camera mounted on the microscope. From well stained slides 200 good specimens of T. danilewskyi FCc-1 were selected. For morphometric parameters, linear measurements in microns and nuclear area in square microns were taken by using Videoplan ( Image Analysis System Kontron Bildanalyse, Germany) . Morphometric measurements of T. danilewskyi strain FCc-1 were compared with reference strain of T. danilewskyi (Caa-1) already described earlier. The data revealed that T. danilewskyi strains FCc-1 and Caa-1 are the same. passages), isolated from experimentally infected juvenile

K1 morphometry, T. danilewskyi strain FCc-1, common carp, species identification, reference strain Caa-1 PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2013-JAPS-415