RT Journal
T1 VECTOR CONSTRUCTION STRATEGIES FOR TRANSFORMATION OF WHEAT PLANT
A1 M. C. Baloglu
A1 A. Battal
A1 G. Aydin
A1 A. Eroglu
A1 M. T. Oz
A1 M. Kavas
A1 H. A. Oktem
A1 M. Yucel
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 23
IS 3
SP 906
OP 912
YR 2013
FD 2013/06/01
DO DOI NA
AB <p>Gene cloning and vector construction for plant genetic transformation are routine procedures in plant functional genomic studies. The availability of effective transformation vector is one of the pre-requisites for plant transformation studies. Here, we describe the construction of a series of transformation vectors through different genetic engineering techniques for wheat transformation. For this purpose, NAC-type transcription factor genes TaNAC69-1andTtNAM-B2 were isolated from T. aestivum and T. turgidum, respectively. Then they were cloned into different types of cloning and expression vectors. Besides traditional restriction enzyme digestion and ligation method, Gateway cloning technology which is a fast and reliable alternative cloning method were used for construction of wheat transformation vectors. The transformation vectors constructed in this study are suitable for use in both particle bombardment (biolistic) and Agrobacterium based transformation protocols for wheat plant.</p>
K1 Traditional gene cloning, Gatewaygene cloning, Vector construction, Wheat transformation
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2013-JAPS-337