RT Journal
T1 AUGMENTATION OF BIOLOGICAL TITER OF FOOT AND MOUTH DISEASE VIRUS IN IN VITRO CULTURE
A1 Q. Akram
A1 K. Muhammad
A1 M. Rabbani
A1 J. Nazir
A1 M. Nawaz
A1 K. Hanif
A1 Z. Shakoor
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 23
IS 3
SP 771
OP 774
YR 2013
FD 2013/06/01
DO DOI NA
AB <p>Foot and Mouth Disease (FMD) virus grew well on BHK-21 cell line. The virus showed poor tissue culture infective dose 50 (TCID50) that was log 103.2&plusmn;0.2 in BHK-21 cell line having Glasgow Minimal Essential Medium (GMEM) without Fetal Calf Serum (FCS). Addition of one per cent FCS in the medium @ one percent increased log 107.1&plusmn;0.2 units of virus TCID50. Incubation temperature of 350 C and 370 C supported the multiplication and maintenance of BHK-21 cells and yielded log106.6&plusmn;0.1 and log107.0&plusmn;0.2units of virus TCID50, respectively. Each serotype of FMD virus showed log106.29&plusmn;0.07 units of virus TCID50 in the stationary monolayer of BHK-21 cells in roux flask (75cm 2), log107.66&plusmn; 0.02 units of virus TCID50 in roller bottles (490cm2) and log108.34 &plusmn; 0.07 units of virus TCID50 on 0.2 g of micro-carriers suspending in 200 ml of the growth medium in stirring bottle. The infectivity titer/TCID50 of each of the virus serotypes was significantly higher in roller bottles than that achieved in roux flasks (p&lt;0.05) and was significantly higher in stirring bottle containing micro-carriers suspending in the growth medium than that of harvested in roller bottle (p&lt;0.05). It is concluded that the infectivity titer of the virus is directly proportional to number of BHK-21 cells in the culture system.</p>
K1 Anchorage dependant BHK-21 cells, roller bottles, infectivity titer, FMD virus
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2013-JAPS-315