RT Journal
T1 PREVALENCE OF AVIAN INFLUENZA VIRUSES IN LIVE BIRD MARKETS OF LAHORE
A1 M. Sarwar
A1 K. Muhammad
A1 M. Rabbani
A1 M. Younus
A1 N. Sarwar
A1 M. A. Ali
A1 A. Ahad
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 23
IS 2
SP 388
OP 392
YR 2013
FD 2013/04/01
DO DOI NA
AB <p>Prevalence of Avian Influenza Viruses (AIV) in live bird markets of Lahore was determined. A total of 1500 cloacal swabs were collected from seven live bird markets of Lahore, Pakistan for surveillance of the viruses. The samples were tested for virus isolation in chicken embryos. Allanto-amniotic fluid (AAF) of each of the embryos was used for Haemagglutination (HA) test. Eighteen (18/1500) samples were positive for HA activity. AIV Antigen Rapid Test Kit differentiated 4 HA virus as AIV and other 14 as Newcastle disease virus. Haemagglutination Inhibition test also proved four HA positive isolates as AIV, while remaining fourteen as NDV, using specific sera. All the AI virus isolates were confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) using a universal nucleoprotein (NP) primer set specific to the virus NP gene and serotype specific primers (H9 and N2) for its sub typing as H9N2.</p>
K1 Avian Influenza virus, H N , live bird markets, RT-PCR
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2013-JAPS-209