RT Journal
T1 DETECTION OF MYCOBACTERIUM BOVIS IN BUFFALOES BLOOD THROUGH POLYMERASE CHAIN REACTION (PCR ) AND TUBERCULIN TEST
A1 A. Khan
A1 Z. I. Chaudhry
A1 A. R. Shakoori
A1 N. Mahmood
A1 M. Ijaz
A1 M. Z. U. Khan
A1 Saima
A1 I. Jan
A1 M. M. Ali
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 22
IS 3
SP 237
OP 241
YR 2012
FD 2012/06/01
DO DOI NA
AB <p>A study was conducted in buffaloes to detect the M. bovis in blood samples through PCR. The PCR assay was described which could detect M. bovis infection in blood samples that were apparently negative in PPD of tubercle bacilli. A total of 100 buffaloes blood samples were tested and 2.0 % buffaloes were found positive for M. bovis infection through single comparative cervical intradermal tuberculin test (SCCIDTT) while 54 % were found positive for M. bovis infection through PCR. The positive cases were analyzed in the background of their history. History revealed that these positive buffaloes were reared in a populated herd for a long time. This study provides an evidence of bovine tuberculosis caused by M. bovis in buffaloes which are main source of milk and meat is South Asia.</p>
K1 bovine tuberculosis, buffaloes, prevalence, tuberculin test, PCR.
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2012-JAPS-3023