RT Journal
T1 MOLECULAR DIAGNOSIS AND CHEMOTHERAPY OF TRYPANOSOMA EVANSI IN NILI-RAVI BUFFALOES AT DISTRICT OKARA (PAKISTAN)
A1 W. Shahzad
A1 R. Munir
A1 M. S. Khan
A1 M. U. D. Ahmad
A1 M. Iqbal
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 22
IS 3
SP 212
OP 216
YR 2012
FD 2012/06/01
DO DOI NA
AB <p>A total of 144 blood samples were collected from apparently emaciated and anemic Nili-Ravi buffaloes in and around Livestock Experiment Station Bahadurnagar Okara during December, 2008 to May, 2009 including all age groups and either sex. They were screened for the presence of trypanosomes through stained (Hemacolor&trade;, Merck) thin blood smears. The cases were confirmed as T. evansi through polymerase chain reaction (PCR). DNA of the trypanosomes was isolated using trizol reagent. The assay employed primers ESAG 6/7 specific for 237 bp fragment from T. evansi genomic DNA which was subsequently electrophorised and analyzed on UV transilluminator. Eight samples (5.5%) were positive microscopically while 16 samples (11.11 %) were found positive through PCR, indicating the specificity and sensitivity of PCR for surveying the disease in epidemiological studies. All the diseased buffaloes were treated with diminazene aceturate (FA.TRY.BANIL R.T.U., FATRO, Italy @ 3.5 mg/ kg body weight). The treated animals were found negative for the parasite both microscopically as well as through PCR, 48 hours post injection indicating the sensitivity of this drug to T. evansi in buffaloes.</p>
K1 T. evansi, Nili Ravi buffalo, PCR, Diminazene aceturate
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2012-JAPS-3017