RT Journal
T1 EFFECT OF ORGANOPHOSPHATE PESTICIDE MALATHION ON IN-VITRO OOCYTE MATURATION, FERTILIZATION AND EMBRYO DEVELOPMENT OF NILI RAVI BUFFALO (BUBALUS BUBALIS)
A1 F. Aziz
A1 Z. Mehboob
A1 S. Jalali
A1 S. A. Shami
A1 I. Z. Qureshi
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 22
IS 3
SP 147
OP 152
YR 2012
FD 2012/06/01
DO DOI NA
AB <p>The present study evaluated in-vitro toxicity of organophosphate insecticide, malathion on nuclear maturation, fertilization and subsequent embryo development of the oocytes obtained from abattoir ovaries of Nili-Ravi Buffalo (Bubalus bubalis). The cumulus-oocyte complexes (COCs) in culture medium were exposed to four different regimens of malathion, 1.56 &micro;mol, 3.12 &micro;mol, 6.25 &micro;mol and 12.5 &micro;mol. Results demonstrated dose dependent significant difference (P &lt; 0.05) in nuclear competence, oocyte maturation and degeneration. Cumulus expansion showed significant increase (P &lt; 0.05) while oocyte diameter increased non significantly (P &gt; 0.05). The COCs that attained nuclear maturation (MII) and were subjected to IVF and, subsequent embryonic development also showed dose dependent non-significant (P &gt; 0.05) decrease in fertilization and embryonic development, however, hatched blastocyst formation was significantly compromised (P &lt; 0.05). The blastocyst development with inner cell mass was again non- significantly different (P &gt; 0.05). The study concludes that malathion may effect quality of preimplantation embryos developed in-vitro from immature oocytes pre-exposed to the pesticide.</p>
K1 Malathion; organophosphorous insecticides; in-vitro maturation; buffalo oocytes
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2012-JAPS-3006