RT Journal
T1 ISOLATION AND BIOCHEMICAL CHARACTERIZATION OF RHIZOBIUM MELILOTI FROM ROOT NODULES OF ALFALFA ( MEDICO SATIVA)
A1 F. Shahzad
A1 M. Shafee
A1 F. Abbas
A1 S. Babar
A1 M. M. Tariq
A1 Z. Ahmad
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 22
IS 2
SP 522
OP 524
YR 2012
FD 2012/04/01
DO DOI NA
AB <p>The Rhizobium a nitrogen fixing bacteria is the essential feature of leguminous plants. Increased cultivation of legumes is essential for the regeneration of nutrient-deficient soils and providing needed nutrients to humans and animals. The present study was aimed to isolate the beneficial nitrogen fixing rhizobium from root nodules of Alfalfa (<em>Medico sativa</em>) plant. Total of Fifty (50) nodules samples were collected equally from five different localities of District Quetta Balochistan, Pakistan and were subjected to culture on differential media Bromothymol Blue (BTB) added Yeast Extract Mannitol (YEM). After series of biochemical and Sugar Fermentation tests twenty Five (25) samples were identified as Sinorhizobium meliloti. The organism was present in all areas. This study confirms the presence of Rhizobia in leguminous fodder in the area.</p>
K1 Root nodules, alfalfa, Rhizobium meliloti, bromothymol blue
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2012-JAPS-247