RT Journal
T1 CALLUS INDUCTION AND PLANT REGENERATION FROM MATURE SEEDS OF SIBERIAN WILDRYE GRASS (ELYMUS SIBIRICUS L.)
A1 K. W. Lee
A1 O. Chinzorig
A1 G. J. Choi
A1 K. Y. Kim
A1 H. C. Ji
A1 H. S. Park
A1 S. H. Lee
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 22
IS 2
SP 518
OP 521
YR 2012
FD 2012/04/01
DO DOI NA
AB <p>In order to optimize tissue culture conditions of Siberian wildrye grass, the effects of plant growth regulators and various carbon sources on callus induction and plant regeneration was investigated with mature seeds. The optimal concentration of 2,4-D for the induction of primary callus from mature seeds was 5 mg/L. The highest embryogenic callus frequency was observed when the mature seed were cultured on MS medium supplemented with 5 mg/L 2,4-D and 0.1 mg/L BA(6-benzyladenine). The highest plant regeneration frequency was observed when callus was transferred to N6 medium supplemented with 1 mg/L 2,4-D and 3 mg/L BA. Regenerated plants were rooted at the highest rate (100%) when transferred onto 1/2 MS medium. Regenerated plants were morphologically uniform with normal growth pattern. A short tissue culture period and regeneration system would be beneficial for molecular breeding of Siberian wildrye grass by the production of transgenic plant.</p>
K1 Siberian wildrye grass, Elymus Sibiricus L., Callus, Plant regeneration, Grassland
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2012-JAPS-246