RT Journal T1 ANTIBACTERIAL ACTIVITY OF INDIGENOUS HERBAL EXTRACTS AGAINST UREASE PRODUCING BACTERIA A1 R. Yasmeen A1 A. S. Hashmi A1 A. A. Anjum A1 S. Saeed A1 K. Muhammad JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 22 IS 2 SP 416 OP 419 YR 2012 FD 2012/04/01 DO DOI NA AB

Aqueous and alcoholic extracts of 14 local herbs (Aloe Vera, Azadirachta indica, Allium sativum, Calotropis procera, Cannabis sativa, Carum capticum, Eucalyptus camaldulensis, Lantana camara, Mangifera indica, Mentha piperita, Nigella sativa, Opuntia ficus indica, Piper nigrum and Zingiber officinalis) and four commercial products (Mentofin, Suduri, Safi, Yucca) were evaluated for their in-vitro antibacterial activity against Proteus mirabilis by serial dilution method. Complete random design (CRD) was followed. It was observed that with reference to rise in pH, Ammonia concentration and urease activity in aqueous and alcoholic extracts of Allium sativum (pH: 8.5560, 8.8480, Ammonia:4.42, 3.52 µg/mL, Urease: 0.009, 0.007 IU/mL respectively) had shown best results as compared to control positive (pH: 9.03, Ammonia: 6.7µg/mL, Urease: 0.013 IU/mL). Alcoholic extracts of Mangifera indica (8.8820, 5.42µg/mL, 0.010 IU/mL), Mentha piperita (8.8880, 4µg/mL, 0.008IU/mL) Carum capticum (8.9540, 4.84µg/mL, 0.009IU/mL) and aqueous extract of Opuntia ficus indica (8.8100, 5.22µg/mL, 0.010 IU/mL) had weak activity against P. mirabilis. Both aquous and alcoholic extracts of Euclyptus camalduensis (pH: 8.91, 8.96, Ammonia: 5.16, 5.06 µg/mL, Urease: 0.01, 0.01 IU/mL) had weak inhibitory effect. All commercial products had shown a strong antibacterial activity (pH: 4.8-6.8, Ammonia: 0µg/mL, Urease: 0 IU/mL). Results of remaining herbal extracts were not significantly different (p< 0.05) from positive control. It was concluded that all products had strong antibacterial activity against P. mirabilis. Mentofin at dilution rate of 1/1000 had shown the best results with optimum inhibitory concentration. Alcoholic extracts of few herbs had shown weak bactericidal activity. These herbs might be effective in in-vivo studies.

K1 Antibacterial activity, Herbal extracts, Urease producing bacteria PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2012-JAPS-229