RT Journal
T1 PRODUCTION OF GOAT POX VIRUS VACCINE FROM A LIVE ATTENUATED GOAT POX VIRUS STRAIN
A1 F. Abbas
A1 F. A. Khan
A1 A. Hussain
A1 F. Ahmed
A1 M. Ahmed
A1 S. Ahmed
A1 M. Z. Ahmed
A1 M. A. Attique
A1 A. Wadood
A1 M. K. Taj.
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 20
IS 4
SP 315
OP 317
YR 2010
FD 2010/08/01
DO DOI NA
AB <p>Kid kidney cells form less than 2 months of age were grown, 80% confluent monolayers were obtained after 10-12 days.&nbsp; &nbsp;Live attenuated goat pox virus vaccine (Gorgan 55, Iranian) was used to infect the confluent monolayers.&nbsp; &nbsp;Eighty percent cytopethic effect (CPE) was obtained on day 5 of virus inoculation.&nbsp; TCID50 calculated for the pool virus was 10 5.5/ ml.&nbsp; Vaccine was found free from any bacterial and mycoplasma contamination. All mice and guinea pigs were found healthy after 21days post vaccinal administration.&nbsp; Vaccinated goats challenged after 14 days with 1 ml of virulent goat pox virus (TCID50 104.5/ml) did not show any unwanted reaction while controls challenged with 1 ml of virulent goat pox virus (TCID50 10 3.5/ml)&nbsp; showed pox lesions and rise in body temperature. The main objective of this study was to produce and study the efficacy of this vaccine.</p>
K1 attenuated, goat pox virus, confluent, monolayer, virulent, challenge
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2010-JAPS-411