RT Journal T1 EFFECTS OF GLYCININ AND b -CONGLYCININ ON INTEGRITY AND IMMUNE RESPONSES OF MOUSE INTESTINAL EPITHELIAL CELLS A1 J. Xu A1 A. Zhou A1 Z. Wang A1 D. Ai JF Journal of Animal and Plant Sciences JO JAPS SN 1018-7081 VO 20 IS 3 SP 170 OP 174 YR 2010 FD 2010/06/01 DO DOI NA AB

Antigen protein contained in soybeans are the major cause of allergic reaction in young animals. In this study, glycinin and β-conglycinin were purified material and experimentally tested at concentration of 0.1, 5, and 10 mg/ml for their effects on integrity and immune responses of mouse intestinal epithelial cells. At 5 mg/ml or above, glycinin and β-conglycinin undermined the integrity of intestinal epithelial cells, inhibited epithelial cell proliferation, and significantly increase the levels of LDH and GOT released to the culture medium. The administration of glycinin and β-conglycinin also significantly promoted the secretion of inflammatory cytokines (IL-2, IL-6, IL-8), a key feature in allergic reactions.

K1 Glycinin, β-conglycinin; intestinal epithelial cells; integrity; cell factor PB Pakistan Agricultural Scientists Forum LK https://thejaps.org.pk/AbstractView.aspx?mid=2010-JAPS-307