RT Journal
T1 ISOLATION AND IDENTIFICATION OF PESTE DES PETITS RUMINANTS VIRUS BY CELL CULTURE AND IMMUNOCAPTURE ENZYME LINKED IMMUNOSORBENT ASSAY
A1 S. Bahadar
A1 A. A. Anjum
A1 M. D. Ahmad
A1 A. Hanif
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 19
IS 3
SP 119
OP 121
YR 2009
FD 2009/06/01
DO DOI NA
AB <p>Thirty tissue samples each of Necrotic debris in buccal mucosa, nasal discharges, ocular discharges and lymph nodes were collected from clinical cases and checked for Peste des Petits Ruminants (PPR) virus using Immunocapture Enzyme Linked Immunosorbent Assay (IC ELISA). The IC ELISA showed presence of PPR antigen 43.3% (13/30) in buccal mucosa samples, 36.6% (11/30) in nasal discharges, 40% (12/30) in ocular discharges and 80% (24/30) in lymph nodes. Overall percentage of PPR antigen in all different samples detected by IC ELISA was 50%. Samples found positive for Vero cell cultures were subjected to antigen detection by using IC ELISA. Out 30 buccal mucosa samples, 30 nasal discharges, 30 ocular discharges and 30 lymph nodes 11, 9, 9 and 22 samples were found positive respectively. Thus an overall 41% samples were found positive by cell culture isolation and IC ELISA. Lymph nodes were found to be the best organ for identification of PPR antigen by IC ELISA.</p>
K1 PPR, IC ELISA, Cell Culture
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=2009-JAPS-309