RT Journal
T1 COMPARISON OF ANALYTICAL SENSITIVITY AND SPECIFICITY OF REVERSE TRANSCRIPTION LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (RT-LAMP) AND REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION (RT-PCR) FOR DETECTION OF LOCAL PESTIVIRUS A
A1 Hammad-ur-Rehman1
A1 A. Ahmad1
A1 M. Rabbani
A1 M. Y. Tipu3­
JF Journal of Animal and Plant Sciences
JO JAPS
SN 1018-7081
VO 30
IS 1
SP 50
OP 57
YR 2020
FD 2020/01/02
DO DOI https://doi.org/10.36899/JAPS.2020.1.0006
AB <p>The main aim of this study was to compare reverse transcription loop-mediated isothermal amplification (RT-LAMP) and reverse transcription polymerase chain reaction (RT-PCR) in order to determine their analytical sensitivity and specificity using local characterized Pestivirus A. In order to compare the sensitivity of RT-LAMP and RT-PCR, serial 10 fold dilutions containing 9.91 x 1010 to 9.91 x 10-2 copies of cDNA of Pestivirus A, were prepared and tested. RT-LAMP proved more sensitive and was able to detect 9.91x100 copies of cDNA compared to RT-PCR (9.91x101). Both RT-LAMP and RT-PCR were found equally specific as no cross reaction with bovine herpes virus was observed.&nbsp; Present study showed that RT-LAMP assay is highly sensitive, specific, rapid and can be used as an alternative to conventional RT-PCR, for the detection of Pestivirus A.</p>
K1 Pestivirus A, Sensitivity, Specificity, RT-LAMP, RT-PCR
PB Pakistan Agricultural Scientists Forum
LK https://thejaps.org.pk/AbstractView.aspx?mid=06-IBC-19-017