CRYOPRESERVATION OF MENTHA PIPERITA L. GERMPLASM AND CONFIRMATION OF GENETIC STABILITY AFTER CRYO-STORAGE Authors: S. Galatali, M. Ceylan, E. Ogur, N. Adanacioglu, E. Kaya Journal: Journal of Animal and Plant Sciences (JAPS) ISSN: 1018-7081 (Print), 2309-8694 (Online) Volume: 33 Issue: 2 Pages: 345-356 Year: 2023 DOI: https://doi.org/10.36899/JAPS.2023.2.0625 URL: https://doi.org/https://doi.org/10.36899/JAPS.2023.2.0625 Publisher: Pakistan Agricultural Scientists Forum Abstract:

Peppermint is an important aromatic-medicinal plant species and it has valuable essential oil contents such as menthol, linalool and limonene. The main purpose of the current study was to optimize a protocol for cryopreservation of M. piperita local cultivars (G-74, Candarli and Gomec). The secondary aim was to investigate the genetic stability after cryopreservation using ISSR marker system. Three different single step freezing techniques were compared for long-term preservation of these local cultivars and the optimum regeneration percentages were acquired by using the droplet vitrification technique. The PVS2 treatments of this technique showed succesful long-term preservation of M. piperita cultivars with regeneration percentages of 72% to Candarli cultivar (60 min PVS2), 52% to Gomec cultivar (75 min PVS2) and 62.5% to G-74 cultivar (30 min PVS2) respectively. The ISSR PCR results showed that the genetic stability from cryopreserved M. piperita cultivars were high. While genetic stability percentage was ~99% for the Candarli cultivar, for the Gomec and G-74 cultivars the genetic fidelity was100%. The shoots that come from the cryopreserved shoot tips showed normal and health rooting, and all of them were also easily adapted to greenhouse conditions.

Keywords: DMSO, encapsulation, ISSR-PCR, PVS2, vitrification