CHARACTERIZATION OF PASTEURELLA MULTOCIDA IN DIFFERENT EGYPTIAN CHICKEN FLOCKS

Authors: WafaaA.AbdEl-Ghany, HananA.Ahmed andAliZ.Qandoos
Journal: Journal of Animal and Plant Sciences (JAPS)
ISSN: 1018-7081 (Print), 2309-8694 (Online)
Volume: 28  Issue: 6  Pages: 1693-1700
Year: 2018
DOI: NA
URL: https://doi.org/NA
Publisher: Pakistan Agricultural Scientists Forum

Abstract:
Inthepresentstudy,phenotypicandgenotypiccharacterizationof Pasteurella multocida(P. multocida)strainsfrom differentEgyptianlayersandbreederschickenflockswascarriedout.Organswerecollectedfrom55chickensflocks represented39layersand16broilersandlayersbreedersinEgyptduringtheperiod2016-2017.Sixbirdsweretakenfrom eachsuspectedflockwithatotalnumber330birdsthatrepresenting234and96freshlydeadlayersandbreederschickens, respectively.Suspectedorganscontaining P. multocidaweresubjectedforisolationandidentificationoftheorganism,as wellastestingofpathogenicityinmice.Moreover,genotypiccharacterizationof P. multocidaisolatewasdoneusing PolymeraseChainReaction(PCR).Capsularandsomatictypingwasdone.Thephenotypicandgenotypiccharacterization resultsrevealedthat6flockswerepositivefor P. multocidaoutof55ones(10.9%).Outof330deadchickens,36(10.9%) werepositivefor P. multocida.All P. multocidastrainswerepathogenicformice.Capsulartypingof P. multocidausing multiplexPCRshowedthatallstrainsweretypeA,whilesomatictypingrevealedpresenceoftypes1and3.Itcouldbe concludedthat P. multocidaiscirculatinginlayersandbreederchickenflocksinEgyptcreatingsevereeconomiclosses. multocida,PCRisused(Townsend et al.,1998,Sellyei et

Keywords: Pasteurella,PCR,LayersandBreeders,Egypt.