POLYMORPHISM OF β-LACTOGLOBULIN IN PRAMENKA SHEEP BREED IN BOSNIA AND HERZEGOVINA Authors: A. Rustempašić, A.Dokso, E.Zečević, A. Hodžić, A.Hrković-Porobija, Z.Sarić, E. Miskoska–Milevska, Z. Popovski, M. Brka Journal: Journal of Animal and Plant Sciences (JAPS) ISSN: 1018-7081 (Print), 2309-8694 (Online) Volume: 28 Issue: 1 Pages: 337-340 Year: 2018 DOI: NA URL: https://doi.org/NA Publisher: Pakistan Agricultural Scientists Forum Abstract:

The aim of this study was to determinate allele frequency β-lactoglobulin in Pramenka breed sheep in Bosnia and Herzegovina. The DNA was extracted from blood of 283 sheep, collected from four flocks around Sarajevo, Bosnia and Herzegovina. The gene β-lactoglobulin was amplified using polymerase chain reaction – restriction fragment length polymorphism (PCR–RFLP) technique. Two genetic variants (A and B) and three genotypes (AA, AB and BB) of β-lactoglobulin have been identified. Genotype frequencies of β-lactoglobulin were with the following values: AA (0.325), AB (0.445), and BB (0.229), while the frequencies of allele A and B were 0.547 and 0.453 respectively. The highest frequency of genotype AA is present in the location Treskavica (38%), genotype AB in the location Vlašić (52%), while the genotype BB is mostly present in location Bjelašnica (29%). The population of Pramenka breed sheep was in Hardy-Weinberg equilibrium. It is expected that in a future the programs in sheep breeding in Bosnia and Herzegovina certainly would take into account the genetic variants of β-lactoglobulin that could contribute to better production and economic benefit.

Keywords: β-lactoglobulin;genetic polymorphism; Pramenka breed