CHANGESINTHEEXPRESSIONOFADIPONECTINANDITSRECEPTORS(A DIPOR1 ANDADIPOR2)INSEXUALLYIMMATUREANDSEXUALLYMATURESHEEPTESTES

Authors: ManBai, LiminSun, JiaZhao, GuishanZhang, WeiMa, LujieXiang, JiarongLi, ChaoJia, andHuaizhiJiang.
Journal: Journal of Animal and Plant Sciences (JAPS)
ISSN: 1018-7081 (Print), 2309-8694 (Online)
Volume: 28  Issue: 4  Pages: 989-995
Year: 2018
DOI: NA
URL: https://doi.org/NA
Publisher: Pakistan Agricultural Scientists Forum

Abstract:
Recentevidenceshaveindicatedthatadiponectinanditsreceptorshaveaneffectonmalereproduction.Theobjectiveof thisstudywastodetecttheexpressionofadiponectin,AdipoR1andAdipoR2insexuallyimmatureandsexuallymature sheeptestesusingqRT-PCRandWesternblot.Theresultsshowedthatadiponectin,AdipoR1andAdipoR2wereall expressed insheep testes. The expressionof AdipoR1 and AdipoR2 mRNAs(p<0.05 and p<0.01) as wellas the expressionofAdipoR1andAdipoR2proteins(p<0.01andp<0.05)insexuallymaturetestesweresignificantlyhigher thanthatinsexuallyimmaturetestes.However,therewasnosignificantdifferenceintheexpressionof adiponectin mRNA(p>0.05)insexuallyimmatureandsexuallymaturetestes,theexpressionofadiponectinproteininsexually maturetesteswassignificantlyhigherthanthatinsexuallyimmaturetestes(p<0.01).Itisthefirsttimetocomparethe mRNAandproteinexpressionofadiponectinanditsreceptorsinsexuallyimmatureandsexuallymaturesheeptestes. and AdipoR2wereexpressedinhumanandratpituitary

Keywords: adiponectin,AdipoR1,AdipoR2,sheep,testes,qRT-PCR,Western-blot