BIOLOGICAL EVALUATION OF LOCALLY PRODUCED RECOMBINANT PHYTASE IN BROILER CHICKS

Authors: F.Sabir, M.Tayyab, A.R.Awan, B.Muneer, A.S.Hashmi, M.Wasim andS.Firyal
Journal: Journal of Animal and Plant Sciences (JAPS)
ISSN: 1018-7081 (Print), 2309-8694 (Online)
Volume: 28  Issue: 3  Pages: 946-950
Year: 2018
DOI: NA
URL: https://doi.org/NA
Publisher: Pakistan Agricultural Scientists Forum

Abstract:
Currentstudydealswiththeproductionoflocallycharacterizedrecombinantthermostablephytasefrom Thermotoga naphthophila(PHY )anditsbiologicalevaluationinpoultrybroilerchicks.ThePHY wasproducedfrom16-literLB TN TN mediumusingBL21CodonPluscellshavingpET-21acontainingphytasegenefrom Thermotoga naphthophila.Atotal of135birdsweredividedinto5groupseachhaving27birds.GroupAservedasnegativecontrol,groupB,CandD weregivenfeedsupplementedwith500,1000and1500IU/kgoflocallyproducedrecombinantphytasewhilegroupE servedaspositivecontrol.Theresultsdepictedthatlocallyproducedrecombinantphytaseshowedsignificanteffecton birdsweightgain,feedintakeandfeedefficiencyratio.Presenceof1000IU/kgofphytaseresultedintheweightgainin rd th th 3 ,4 and5 weekoftrialfrom504.77±1to533.53±1.4,767.9±2to823.73±2.84and999.8±7.66to1122.8±2g respectivelywhencomparedwiththecontrol.ThegroupB,CandDshowedanaveragefeedintakeof2710,2780and 2790gascomparedto2669gforgroupA.Thesupplementationof1000IU/kgoflocallyproducedphytaseinfeed resultedintheimprovementoffeedconversionratiofrom2.6to2.4.ThestudydemonstratedthatPHY issuitablefor TN poultryfeedindustryanditsdomesticproductionwillcontributeintheeconomicavailabilityoflocallyproducedphytase forthepoultryfeedindustry. TN supplementationinmonogastricdiets.Thefeedpelleting

Keywords: PHY ,PoultryTrial,RecombinantThermostablePhytase, Thermotoga naphthophila,Phytate