ACUTE DERMAL AND OCULAR IRRITATION TESTING OF GRAPE FRUIT SEED EXTRACT IN NEW ZEALAND WHITE RABBITS Authors: Jae Young Kim, Jae-Suk Choi Journal: Journal of Animal and Plant Sciences (JAPS) ISSN: 1018-7081 (Print), 2309-8694 (Online) Volume: 28 Issue: 2 Pages: 485-490 Year: 2018 DOI: NA URL: https://doi.org/NA Publisher: Pakistan Agricultural Scientists Forum Abstract:

We investigated the acute dermal and ocular irritation potentials of grapefruit seed extract (GSE) in New Zealand white rabbits. In the dermal irritation test, erythema, eschar, and edema formation were observed after 24 h of GSE treatment, and the skin returned to normal after 72 h. The dermal primary irritation index of GSE was 0.25; therefore, it was classified as a mild irritant. In the ocular irritation test, there were no clinical signs related to the application of GSE; therefore, it was classified as a non-irritant according to the Draize scoring system. These results suggest that GSE is mildly or not irritating to the skin and eye, indicating that it can be used in cosmetic and pharmaceutical applications with no serious toxic effects at moderate doses.

Keywords: Grapefruit seed extract (GSE), Dermal irritation test, Ocular irritation test